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KMID : 1123920090230010206
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Korean Journal of Oriental Physiology and Pathology
2009 Volume.23 No. 1 p.206 ~ p.213
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Sophora Flavescens Suppresses Degranulation and Pro-inflammatory Cytokines Production through the Inhibition of NF- (p65) Activation in the RBL-2H3 cells
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Lyu Ji-Hyo
Park Sang-Eun
Hong Su-Hyun
Kim Dong-Kyu
Ko Woo-Shin
Hong Sang-Hoon
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Abstract
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Sophora flavescens, as a traditional herbal medicine, has been used to treat with a variety of disesases, In previous reports, S. flavescens and sophoraflavanone G (a prenylated flavonoid from S. flavescens) inhibited cytokines productions in LPS-induced Raw 264.7 macrophages cells and BV2 microglial cells. We examined on the anti-allergic effect of S. flavescens on the PMA plus A23187-induced rat leukemia (RBL-2H3) cells. S. flavescens inhibited the release of -hexosaminidase and productions and expressions of tumor necrosis factor (TNF)-, interleukin (IL)-4 and cyclooxygenase (COX)-2 in a dose-dependent manner on stimulated RBL-2H3 cells, however, S. flavescens not affect cell viability. The protein expression level of nuclear factor (NF)- (p65) was decreased in the nucleus and suppressed the degradation of inhibitory protein protein, the activation of extracellular signal-regulated kinases (ERK) mitogen-activated protein kinase (MAPK) by S. flavescens. These results suggest that S. flavescens could be involved anti-allergic effect by control of (p65) translocation into the nucleus through inhibition of degradation and suppression of pro-inflammatory cytokines expression.
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KEYWORD
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Sophora flavescens, pro-inflammatory cytokines, nuclear factor kappa B, Inhibitor kappa B, ERK Mitogen-Activated protein kinase
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